Suppressing conditions decrease physiological demands by slowing the growth rate and thereby bring the cells back to normal homeostasis at elevated temperatures. Because Sec9-4p and Sec9-7p have differing propensities to form SNARE complexes, this constitutive function of SEC9 may be independent of SNARE complex formation. Further characterization of the sec9 suppression phenotype and molecular genetic studies could be utilized to identify this novel function. During somitogenesis, the rate of ribosome production may vary between organs and, because of its large size and rapid development, this rate may be CHIR-99021 highest in the brain. At 24 hpf, the expression pattern of many assembly factors and processing proteins, such as bop1, rrs1, and bap28, show a spatial distribution, with the highest concentration in the brain region, supporting the notion that ribosome production is highest in the brain. Therefore, absence of L11 leads to impaired ribosome synthesis, which results in phenotypes specific to the brain region. As mentioned previously, a number of cellular studies have shown RPs as negative regulator of p53 because of their MDM2 binding property. A decrease of these RPs should allow an increase of free MDM2, thereby decreasing the levels of p53. However, our results indicate that in zebrafish loss of L11 individually or in combination with other two RPs , actually increases the p53 activity, rather than decreasing it. Recently, it has been shown that RP mutant zebrafish which develop MPNSTs , fail to synthesize p53 protein, but this observed loss of p53 is not due to the L5/L11/L23-MDM2 regulation. Therefore, it seems likely that some other mechanism of p53 activation exists in the cell which links RPs to ribosomal biogenesis stress. We speculate that in a model system like zebrafish, loss of L11 would affect its primary function, namely participation in ribosome assembly. Accumulation of defective ribosomes due to RP deficiency would be sensed as a stress signal, presumably a nucleolar stress, leading to programmed cell death. Acute respiratory infections and diarrhea are the leading causes of childhood morbidity and mortality worldwide, each resulting in an estimated nearly 2 million deaths annually. The diagnosis of respiratory and gastric/digestive infections is complex, due to the wide range of potential pathogens that can present with the same clinical symptoms. In addition to the many known causes of these infections, it has been suggested that unrecognized infectious agents, including viruses, remain to be discovered. Capillary leak may be transient, as seen in response to histamine or prolonged as seen in response to thrombin. Extensive leakage often occurs in intensive care patients and is thought to be caused by the exposure of endothelial cells to activated coagulation factors plus pro-inflammatory stimuli. This is because active Rho activates Rho kinase which phosphorylates MLCpho, a negative regulator of myosin.