The full genome for L. sericata is not publically available, only the mitochondrial genome is published. Some short DNA sequences have also been released for use in AS 2444697 species identification in forensics. Due to the importance of this species, the transcriptomes of the developmental stages and dissected salivary glands have recently been published. An expressed sequence tag transcriptome has also been assembled for L. cuprina. Gene expression analysis of Lucilia has already shown great value as it is accurate in developmental stage estimation for use in forensics. The small RNA profiles in multiple flying insects such as Drosophila, honey bee and mosquito have recently added to the better understanding of their development as well as ability to transmit various diseases as vectors. Therefore, to broaden the biological knowledge of L. Sericata we have performed small RNAsequencing on their larvae tissues and ES. When the data was matched to known small RNA databases we identified both common and tissue specific RNAs, derived from various families of annotated small RNAs. The abundant small RNAs were then were assayed across developmental stages of L. sericata and validated in the dissected tissues by droplet digital RT-PCR. The aim of this study was to use small RNA-sequencing technology to identify the profiles in a range of medicinal maggot tissue samples and in its ES. The standard for analysis of such data is to initially map the reads to the genome of interest before further analysis, discarding any which are unmapped. In our case this was not possible due to lack of a complete published genome. We note therefore that this dataset is limited to common subcategories of small RNAs from orthologous insects. However because of our non-genome-mapping approach to the analysis we were able to identify small RNAs in an unbiased manner, including both fly and bacterial sequences. The RNA component present in ES appears to be derived from all its potential tissue sources. In terms of the overall RNA profiles, tRNA and miRNA patterns, the SG and CROP source libraries are most similar to one another, possible due to the close proximity of these tissues in the maggot foregut. Small RNA-seq of L. sericata tissues and ES highlighted the presence of a varied profile of commonly annotated small RNAs, derived from insect, bacterial and food sources. The surface bleaching of eggs and larvae into medicinal-grade maggots, did as expected and removed a large proportion of bacterial burden; however the database read matches still identified bacterial RNAs in the tissues and ES. The bacterial content was highest in the crop, a food CDP 840 hydrochloride storage organ, which had almost 10-fold more bacterial RNA reads than the gut sample. The crop is where pathogenic bacteria are killed so that the gut remains for the most part, sterile.