Considering the annotation results of the transcriptome data and the reported results, the possible biosynthetic pathway of KGM and Arecaidine propargyl ester hydrobromide starch in konjac leaf was constructed. Among these transcripts, the mRNA sequences of fructokinase and cellulose synthase-like D were reported for the first time, indicating that the corresponding genes of the two enzymes were present in A. konjac and A. bulbifer. Nevertheless, enzyme activities should be confirmed by conducting further investigations. In the known glucomannan biosynthesis pathways, only GDP-D-pyrophosphorylase is absent in the leaves of Amorphophallus. And GGP is also not found in konjac corms. Heller et al. found UDP-glucose, ADP-glucose and GDP-mannose in konjac corms, but no GDP-glucose. It seems there is little probability of the glucose units in KGMs obtained from GDP-glucose. The possible way of KGM synthesis might be GDP-mannose and UDP-glucose was catalyzed by CSLD proteins. This deduction needs the further experiments. In the expressed genes, the numbers of the corresponding transcripts of Ifetroban sodium various functional genes differed significantly. For example, PGI exhibited only one type of transcript with a significantly higher expression level in A. bulbifier than in A. konjac. PMI showed only two types of transcripts with a significantly lower expression level in A. bulbifier than in A. konjac. As GDP-mannose is synthesized from mannose-1-phosphate, two types of GDP-mannose pyrophosphorylase are involved based on the substrate type: type I uses GTP and mannose-1-phosphate as the substrates and type II uses GDP and mannose-1-phosphate as the substrates. In this study, the corresponding transcripts of both types of enzymes were present in Amorphophallus leaves, but type II revealed only one transcript with a low expression, indicating that GMPP type I was the main enzyme involved in the catalytic synthesis of GDP-mannose. The starch is categorized into amylose and amylopectin. The synthesis of plant amylose is catalyzed by granule-bound starch synthase ; amylopectin synthesis can be synergistically catalyzed by soluble starch synthase, starch branching enzyme, and debranching enzyme. The corresponding transcripts of these four enzymes localized in the chloroplasts were found in the transcriptome of Amorphophallus. GBSS exhibited fewer transcripts but higher expression levels. SSS and SBE showed higher numbers of transcripts. Significant differences in expression levels were observed between various transcripts.