As expected, real-time PCR and western blot assays detected that the expressions of those cell cycle regulators were significantly altered following anandamide treatment. Flow cytometry assays further confirmed that anandamide induced G2/M cell cycle arrest in gastric cancer cells through active G2/M checkpoints. This represents the first time that the cell cycle redistribution was detected in gastric cancer cells after being treated with anandamide directly and separately. Additionally, the results indicated that the B-terms could potentially function to mediate the effectors between the disease and the discovery targets. For biological investigators, keeping up-to-date with current published research is a critical component of any investigator��s job description, and nearly every published article is an opportunity to find novel links between drug and disease. However, the current volume of available biological science is enormous. Using the informed traditional search, BIO investigators may observe BMS-870145 limited links between a drug/molecule and a disease, but may not recognize the larger environment through which the relationships operate, nor identify other potential relationships within that environment. Therefore, information sciences and retrieval are very useful tools for biological scientists. Swanson��s literature-based discovery is focused on resurrecting previously published but neglected hypotheses. If a direct connection that seems to be neglected is detected, then the work of resurrection turns out to be of analyzing pathways or mechanisms that might not be known. In other words, Swanson��s literature-based discovery methodology not only mines data for possible interactions between disease and disease, disease and drug, or disease and molecule, but also provides us with the potential to observe the larger background behind these direct links, like the molecular pathway network in our study suggesting a possible link between gastric cancer and anandamide action. By analyzing data in this context, we obtained the interactions and the mechanisms between seemingly unrelated topics and their clinical importance and significance. Therefore, swanson�� s literature-based discovery is an effective tool to seek connected existing knowledge from empirical results by bringing to light relationships that are implicated and yet “neglected”. We also encountered some challenges in the discovery process.