Strains with a modified translation accuracy often displayed aminoglycoside sensitivity. We thus investigated the resistance/ sensitivity of the IMPQ strain against various antibiotics. We tested antibiotics targeting different translation steps, such as the aminoglycosides: G418, paromomycin, ribostamycin, tobramycin, neomycin and kanamycin, as well as DNA damaging agents like bleomycin and phleomycin. Exponentially growing FS1 and IMPQ strains were serially diluted and spotted onto rich medium containing different concentrations of each indicated antibiotics. Results highlighted two interesting points. First, the IMPQ strain appeared to be sensitive to the G418 and paromomycin aminoglycosides. At 250 mg/mL paromomycin, growth of the IMPQ strain is already inhibited, while at 500 mg/mL, no growth was observed. In the same conditions, growth of the FS1 strain was not impaired. Second, an unexpected high sensitivity to bleomycin and to the closely related CK-548 phleomycin was observed for the IMPQ strain. The former result was not CGS-9343B surprising as G418 and paromomycin target the ribosome and the IMPQ strain harboured defects in the ribosome biogenesis. The latter was more intriguing, since bleomycin and phleomycin are anti-tumoral agents known to induce various types of DNA damages. To verify the antibiotic sensitivity phenotypes were directly related to the expression of the mutant imp3Q allele, the IMPQ strain was transformed with a plasmid-encoded wild-type IMP3 gene, and the phleomycin assay was reproduced. As shown on Figure 5B, the presence of a wildtype Imp3 protein restored antibiotic resistance of the IMPQ mutant strain. It should be noted that the assays were made on rich medium, so a non negligible proportion of IMPQ cells may have lost the pCEN-IMP3 plasmid, explaining why a wild-type growth was not totally restored. Indeed each time the complementation was assayed under selection to retain the plasmid, the IMPQ sensitivity phenotype was quite fully corrected. We can therefore conclude the antibiotic sensitivity phenotypes of the IMPQ strain were directly dependent on the partial loss of function of the Imp3 protein. Here, we took advantage of a viable mutant allele of the essential IMP3 gene that permits cell growth to investigate the Imp3p functions.