Secondly, transformation oculd induce chromosome rearrangements such as deletion, translocation,Pazopanib and inversion during transgene insertion. Finally, transgene insertion is not a precisely controlled process which could be the reason that transgenic plants with unexpected phenotypes are generated in the first place. Previously, two tomato genes induced by nutrient stress treatments were identified using cDNA arrays, which putatively play a role in plant mineral nutrition uptake or utilization. When antisense constructs for the two genes were transformed into tomato plants, one dominant flower mutant was identified from transformation of each construct. While flower structural changes can be caused by mutations in the MADS-box gene family, it is unexpected that antisense to two nutrient stress induced genes would cause mutation in flower structure. It is possible that the mutations could be induced by the transformation process itself. Standard protocol was followed for tomato transformation. Micro-Tina tomato seeds were sterilized and sown on Murashige and Skoog medium with vitamins. Five to seven day-old cotyledons from the seedlings were cut at the petioles and at the tips. The explants were incubated upside down on MS plates with PCI-32765 appropriate vitamins and hormones at room temperature for overnight. Agrobacterium tumefaciens GV3101 strain containing a gene construct was cultured on the same day for transformation of these explants the next day. The explants were added to 20 mL of Agrobacterium cell and incubated for 15 minutes with periodic shaking. The explants were then returned to their plates upside down, sealed with micropore tape and incubated at room temperature for two days in subdued light. After this, the explants were transferred into regeneration media to allow for regeneration of shoots. As soon as shoots appeared, they were transferred to rooting medium. After the shoots developed adequate roots, they were transplanted into greenhouse. Age-Related Macular Degeneration is the leading cause of blindness among the elderly in the United States. Due to the aging population, the number of people with advanced AMD will increase from 1.75 million now, to 3 million by 2020.