Ribonuclease A is a pancreatic ribonuclease that cleaves single-stranded RNA. This enzyme has played a crucial role as a model system in studies of protein L-Ornithine structure, folding and unfolding pathways and enzyme catalysis. Lysozyme is part of the innate immune system. Reduced lysozyme levels have been associated with bronchopulmonary dysplasia in newborns. Children fed infant formula lacking lysozyme in their diet have three times the rate of diarrheal disease. Since lysozyme is a natural form of protection from gram-positive pathogens like Bacillus and Streptococcus, a deficiency due to infant formula feeding can lead to increased incidence of disease. Whereas the skin is a protective barrier due to its dryness and acidity, the conjunctiva is, instead, protected by secreted enzymes, mainly lysozyme and defense. Nano-biotechnology is a promising and interdisciplinary research involving chemistry, physics, biology and medicine. Mebhydrolin napadisylate Silicon nanomaterials are a type of important nanomaterials with attractive properties including excellent electronic/ mechanical properties, favorable biocompatibility, huge surfaceto-volume ratios, surface tailorability, improved multifunctionality, as well as their compatibility with conventional silicon technology. Consequently, there has been great interest in developing functional silicon nanomaterials for various applications ranging from electronics to biology. Elucidating protein orientation on nanoscale surfaces has important implications for integrating proteins into micro and nanofabricated devices. Applications include biosensing, actuating of microelectromechanical systems, and tissue engineering, as well as screening tools for drug discovery and basic biological research. Although the literature is replete with reports on the structure, stability, and activity of proteins adsorbed onto nanomaterial surfaces, very little is known about exactly how a protein interacts with a surface and how its orientation is governed by the size, shape, and chemistry of the surface.These are major questions that must be addressed to design both nanoscale surfaces and proteins to achieve optimal conjugate functionality.