As observed in this study, GLG1 was concluded to be significantly associated with T category cancer with regards to invasion depth. It should be noted that GLG1 may be associated with invasion potential of CSCs via FGFR signaling. Vacuolar protein sorting plays a crucial role in the trafficking of molecules between cellular organelles, such as through the trans-Golgi network. The mutation in this gene causes the autosomal disorder characterized by progressive neurodegeneration. A past study found that frameshift mutations of VPS genes,Gypenoside-XVII along with loss of expression of VPS13A proteins, are common in gastric cancers with high microsatellite instability and suggests that these alterations may contribute to the development of cancer. VPS is involved in cancer-related cellular mechanisms, such as proliferation. As observed in this study, VPS13A was expressed in SP cells and was concluded to be associated with a T category cancer and lymph node metastasis of gastric cancer. It should be noted that VPS13A may be associated with the invasion potential of CSCs. DCTPP1 is expressed in the nucleus of various cancer cells. Zhang et al. suggested that the accumulation of DCTPP1 in the nucleus of tumor cells might be sufficient for maintaining proper DNA replication needed in order to fulfill the requirement for survival and proliferation of the cells. In conclusion,Gypenoside-XLIX DCTPP1 was determined to be significantly associated with metastatic activity. It should be noted that DCTPP1 may be associated with the DNA replication of CSCs. HSPA9 and HSPA4 were concluded to be associated with the invasion and metastatic activity of gastric cancer. These two proteins are members of the heat shock protein -70 family of chaperones, and HSPA9 is the major protein in the mitochondria. Some studies have suggested that HSPA9 and HSPA4 are relevant to cellular apoptosis and promoting proliferation; HSPA9 is over-expressed in colon and hepatocellular carcinomas, while HSPA4 is over-expressed in breast, colon, ovarian, and pancreatic cancers. The HSPA4/HSPA14 axis induces the migration, invasion, and transformation of cancer cells.