F-actin architecture in human Tienilic Acid outflow pathway cells in situ differs between normal and glaucoma eyes, glaucomatous tissue showing a more ����disordered���� actin architecture overall. TM and its endothelial cells, as well as SC Schlemm��s canal and the lining endothelial cells, undergo deformation and stretching with changes in intraocular pressure. The above reported results could confirm the hypothesis of the ����mechanical theory���� of Wise and Witter, neverthenless also the ����biological theory���� and the ����repopulation theory���� are almost supported by our findings. The expression of 9 genes characterizing neural tissue, as inferred from Swissprotein annotation, was well detectable expressed in TM cells and modulated by SLT treatment. These genes are identified by ��*�� in Table S1. This finding indicate that similarities at postgenomic levels exist between TM and other neural tissues kb-NB142-70 recognized as POAG targets such as optic nerve head and geniculate ganglion. TM cells have a neuro-ectodermic origin, expressing, at least in part, a neural-like phenotype TM cells derive from mesenchymal cells of the neural crest. This may explains why the SLT increases also the genes expression of typical neural tissues, and further confirm the similarity between TM and neural tissue previously reported comparatively analyzing proteome in TM and optic nerve head. Furthermore, neural proteins have been specifically detected in the aqueous humour of glaucomatous patients as resulting both from TM and optic nerve head damage and these molecules may be involved in the normal formation and function of these tissues. The similarity between TM and neural tissue is important for glaucoma pathogenesis because they can share common pathogenic mechanisms. The herein reported results provide evidence that glutamate cytotoxic effects representing a major pathogenic element for nerve tissues targeted by glaucoma, are also important for TM homeostasis and may be modulated in TM by SLT treatment. A trend to decreasing TM cell to the adverse glutamate effects was detected.