Finally, recombinant poxviruses vectors currently represent highly attractive delivery systems for vaccination against a variety of infectious diseases as well as oncolytic agents against a wide range of different cancers. There is however still a need to improve the safety, immunogenicity and/or oncolytic activity of existing poxvirus based vectors if their full therapeutic potential in the clinic is to be realized. The reduced viral spread, mortality and the ability of mice to eliminate the DF11L virus from their lungs suggests that deletion of the F11L gene in existing vaccinia vectors may help to improve their safety and/or therapeutic potential as oncolytic agents by limiting the spread of infection. Hepatocellular carcinoma is one of the most aggressive tumors making it the third leading cancer-caused deaths worldwide. A majority of HCC treatment failures arise from Sulfadiazine vascular invasion, metastasis, and recurrence after surgical resection. Similar to other cancers, HCC metastasis is a multistep process that involves tumor cell proliferation, invasion, dissemination, immune evasion, and growth at distal sites. Alterations in cancer cell and extracellular matrix interactions in the tumor microenvironment are essential to initiate the process of metastasis._ENREF_7 Recent studies have highlighted the importance of glycosyltransferases in regulating cell-ECM interactions through modulation of integrin functions. However, the contributions of O-glycosylation in the interaction between HCC cells and the ECM have long been overlooked in the past. Iopamidol Mucin-type O-glycosylation is the most common type of Oglycosylation, and it modulates diverse functions of membranebound and secreted proteins. Mucin-type O-glycans are formed when N-acetylgalactosamine is added to a serine or threonine residue to form a GalNAca1-Ser/Thr structure. Core 1 b1,3-galactosyltransferase transfers galactose to the Tn antigen forming the Galb1-3GalNAca1-Ser/Thr structure. The T antigen is a precursor for subsequent elongation of mucin-type O-glycans.