The efficiency of transgenesis and germline transmission was similar to what is currently observed in our rabbit transgenesis facility and led us to suppose that the transgenes were not deleterious for the survival of the rabbits. This suggests that the shRNA produced by the rbapobec1-shRNA transgene targeted the rabbit APOBEC1 gene probably through a RNA interference mechanism. Unfortunately, no antibody was available to detect by Western blot the rabbit APOBEC1 protein in intestinal cell extracts. Thus we are unable to confirm that the level of rabbit APOBEC1 enzyme was lower in rbapobec1-shRNA transgenic animals than in wild type ones. In numerous mammals, it has been already reported that the APOBEC1 induced APOB mRNA editing introduces a STOP codon in the APOB mRNA. The indirect regulation of downstream genes could be Palonosetron hydrochloride through the transcription control mediated by transcription factors or proteins with binding activity among those directly regulated by GRF1 or GRF3. Consistent with this interpretation, genes coding for transcription factors or proteins with binding activity represent up to 39% of the GRF1potential direct target genes and up to 35% of the GRF3- potential direct targets. This hypothesis is developed based on our data showing that the frequency of known cis elements is more Flumethasone abundant in the negatively regulated targets relative to the upregulated targets.One of the most common approaches to identify target genes of the transcription factors involves comparison of the genome-wide transcript profiles of transgenic plants overexpressing transcription factors and the corresponding wild types allowing the identification of genes that are significantly altered as a result of the increased expression of the transcription factors. An alternative approach relies on the comparison between the transcriptome of mutants and wild-type plants.