In a study by Raham and coauthors who used extraction and purification methods and a GA model. In addition, these authors identified the corresponding candidate proteinsand confirmed their presence in the lung tumors by immunochemistry. Microflex LTmass spectrometer laser is a bench disposable material with integrated analysis software that can be easily installed in the operating facilities. The novelty here is that the complete sample treatment process, including tissue dispersion, sample material deposition on the matrix and analysis, does not require technical expertise and could be learned by any paramedical personnel. We used two third of our samples for building the prediction model whereas equal or lower numbers are commonly used for training sets compared to validation sets. This was justified by the heterogeneity of our Cancer population with the aim to increment the training set to obtain a large representation of reference cancerous spectra. Finally, our Blinded set population size was higher than previously published with MALDI-ToF MS on lung tissue. In contrast with our good diagnostic performance in classifying a sample as Cancer versus Non-tumor, we obtained low Compound-K performances for the Primary versus Metastasis subclasses. We think that the large diversity in metastasis subgroups contrasting with the low number of samples analyzed in this subclass could be responsible for a low performance random mathematical model. We hope that incrementing the training cohort with Metastasis would lead to finding a GA model with better diagnostic performance. Adopting complementary and/or alternative exatraction/solubilization methods would improve the yield of detecting m/z peaks. However, increasing preparation step should be balanced with regard to the application of this tool in clinical settings. At this stage of the work, we think it could be possible to give a result in less than 30 minutes, thus determining whether a sample is cancerous or not with a simplified and rapid approach for whole proteomic tissue analysis that could be easily used as a diagnostic aid during routine surgical procedures. The ability to have information reliably confirmed on-theater versus using frozen biopsies could have major implications for the CAY10505 management of patients with tumors. According to the molecular weight markers in the gel-filtration column chromatography, the molecular mass of the stimulatory factor with a low molecular weight was 66 kDa, and analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresisrevealed that the major component of the fraction was a 66-kDa protein. Serum albumin is the major component of serum and its molecular weight 
is 66 kDa. We used purified bovine serum albumin to examine whether serum albumin has colony-spreading stimulatory activity. Purified albumin stimulated colony-spreading activity. Because the purified albumin fraction contained other high molecular weight proteins, we performed ion-exchange column chromatography to examine the coincidence of albumin with colony-spreading activity. In DEAE cellulose column chromatography, the stimulation of colonyspreading activity indeed coincided with the presence of albumin and did not coincide with the presence of several faint high molecular weight proteins. As fatty acids associate with albumin in serum, we next examined whether fatty acid-free albumin enhanced colony-spreading activity. Fatty acid-free albumin stimulated colony-spreading.