A similar phenomenon was reported for inhibitors of the QseC membrane histidine kinase in Salmonella typhimurium and F. tularensis. The addition of LED209 did not influence the growth of Salmonella typhimurium in vitro, while it diminished the expression of virulence genes by 3-fold. This small decrease in the expression of the sifA pathogenicity gene, which is required for the establishment of S. typhimurium in the host, was sufficient to reduce bacterial counts in the liver and spleen of infected animals by 10-fold. Similarly, LED209 reduced the expression of FPI genes iglC and pdpA in F. tularensis by 3-fold in vitro, while clearing the infection in vivo. 4-(Benzyloxy)phenol quinacrine has been used as an antimalarial agent in humans, as an in vitro anti-prion agent, and as a neutralizing agent for Bacillus anthracis. While most research in these systems is oriented to isolate compounds with better affinities and lower host toxicity, Folinic acid calcium salt pentahydrate scarce information is available regarding the specific residues involved in such interactions. Quinacrine disrupts protein-protein interactions in the anti-apoptotic member Bcl-xl, by specifically binding in the hydrophobic grove, competing with the regulatory peptide BH3. As a desired result, apoptosis was induced in cancer cells. To determine the specific residues involved in the binding to quinacrine, a model of the tertiary structure of the MglA/SspA complex was built. Subsequently, site directed mutagenesis was performed in the heterodimer interface, where several residues were 
identified that affected the binding of quinacrine. Mutations to residues Y63 in Ft-MglA, and K97 in Ft-SspA had the greatest effect on the binding of quinacrine, as determined in the two-hybrid system. These results suggest the importance of these residues during interactions of the complex with quinacrine. However, the putative use of quinacrine as a therapeutic agent for the treatment of tularemia disease would require further chemical engineering to improve the affinity for the Ft-MglA/Ft-SspA complex. Recently, Mays et al. have used chemical synthesis to improve the antiprion activity of quinacrine derivatives that bind PrP with higher affinity. Based on the results obtained here, we propose the use of quinacrine as a chemical probe to uncover biologically relevant molecules that may modulate Ft-MglA/Ft-SspA activity, by binding in the heterodimer interface. In addition, it is also an important ingredient in the traditional medicine in treating wounds, headaches, indigestion and rheumatism. During the whole growth season from March to October, Sagittaria trifolia, like most wetland species, is grown in the shallow water, such as the pools, water gardens, tanks or tubs in the greenhouse, which indicates that the plant has developed mechanisms of surviving in the submerged environment. For the regeneration of this plant, bare root stocks or seedlings are directly planted into wetland soil. Several buds from the main stem develop into stolons, and the corms are formed at the tips of each stolon. Actually, corm, tuber, rhizome and bulbs are kinds of underground stems, and work as storage organs. These are storage units for food that provide the plants with the energy for growth, blooming, and completing their lifecycle. The process of corm formation can be classified into three stages: induction, initial swelling, and swelling stage. Stolon tips grow radially in the induction stage. In the second stages, longitudinal growth of stolon stops and its tips swell. At this swelling stage, great amounts of carbohydrates are synthesized in the storage.