Thus, Th1/Th2 ratio was restored to close to the normal level by mangiferin at 200 mg/kg. These findings strongly suggest that mangiferin could restore the balanced of Th1/Th2 cells in asthma. STATs are a group of transcription factors that transmit signals from the extracellular milieu of cells to the nucleus. They are pivotal for the signaling of many cytokines that are Enzalutamide mediators of allergic inflammation and impact various cell types critical to allergy including lymphocytes, mast cells, eosinophils, dendritic cells, and epithelial cells. It have been confirmed that activation of STAT4 and STAT6 is pivotal in Th0 cell differentiate along the Th1 and Th2 pathway, respectively. The STAT6-signaling pathway is mainly induced by IL-4, which induces STAT6’s homodimerization and subsequent translocation into nucleus, and rapidly induces the expression of GATA-3. Expression of GATA-3 is followed by induction of the transcription factor c-MAF, a potent IL-4 gene specific activator. This forms a positive feedback loop for IL-4 induction of GATA-3 and Th2 differentiation. Whereas, the STAT4-signaling pathway is primarily induced by IL-12, and activation of STAT4 induces the expression of T-bet, which activates the IFN-c gene by chromatin remodelling, leading to secretion of IFN-c and increases expression of the IL12Rb2 chain, further enhancing IL-12 signals.. Dysregulation of STAT4/6 signaling has been involved in allergic asthma, therefore, highlighting the importance of these ubiquitous molecules in allergic asthma and the potential of these pathways as a target for therapeutic intervention. It remains unclear whether the protective effect of mangiferin against asthma is associated with STAT4/6signaling pathway. To address these issues, the activity and expression of STAT4, STAT6, GATA-3 and T-bet were detected by western blot and IHC in repeatedly OVA-challenged mice. We found that the levels of p-STAT6 and GATA-3 in the lung tissues were markedly inhibited by mangiferin. Moreover, the expressions of p-STAT4 and T-bet were slightly enhanced by mangiferin. This result suggests that mangiferin mainly inhibits the STAT-6 signaling pathway and consequently modulates the imbalance of Th1/Th2 cells differentiation. Poliomyelitis is caused by the polio virus, an RNA virus that can colonize the gastroenteral tract which may lead to an acute, viral, infectious disease that spreads from person to person, primarily via the fecal-oral route. In 1988, the World Health Assembly resolved to globally eradicate poliomyelitis. The initial objective, the end of polio by 2000, has proven more difficult than originally envisioned and polio still exist in countries such as Afghanistan, Nigeria and Pakistan. However, due to great efforts the number of polio cases has decreased to a level where full eradication within a decade or two is a realistic goal. Two vaccines exist against polio; Inactivated polio Vaccine and Trivalent live Oral polio Virus. tOPV with attenuated Sabin strains of poliovirus types 1, 2 and 3, can immunize or boost immunity of close contacts through secondary spread, and is inexpensive and easy to increased the frequencies of Th1 cells.