Chemokines are a subset of cytokines that selectively regulate the recruitment of leukocytes to inflammatory sites. These receptors are classified into different families according to the type of chemokines they bind. CXC chemokines are especially involved in migration of polymorphonuclear leukocytes, and the prototype of this group is interleukin-8, which binds to the CXCR1/2 receptors. Of note, it has been demonstrated that paraquat is able to activate a set of genes related to immune response, according to evaluation of the human keratinocyte cell line HaCaT. In 1998, White et al. described the first non-peptide, selective, and competitive CXCR2 receptor antagonist, named SB225002, which was found effective in preventing a series of IL8-mediated inflammatory responses, both in vitro and in vivo. By using an in vivo model of mouse colitis induced by 2,4,6trinitrobenzene sulfonic acid, Bento et al. demonstrated that intraperitoneal administration of SB225002, in doses as low as 1 mg/kg, was able to prevent several inflammatory parameters, such as neutrophil migration to the colon tissue. Furthermore, i.p. treatment with SB225002 resulted in a marked and long-lasting inhibition of acute or chronic nociception in mice. Given its role in viral particles transport, the cellular compartments in which US9 accumulates and the contributions of US9 hydrophilic/hydrophobic domains to its localization have been widely studied. Early experiments with PRV US9 and its homologs from other alphaherpesviruses demonstrated that US9 mainly accumulates to the Trans Golgi Network but it is also detected in distal intracellular AbMole BioScience Life Science Reagents regions as well at the plasma membrane, which is in agreement with its role in virus transport. These studies utilized specific antibodies against the viral protein and Hemo-Agglutinin or GFP tags. Notably, in the context of viral infection, GFP-US9 can functionally substitute wild type US9, and has been used to trace protein localization in infected cells.