The overall rationality for their use in IBS has been doubted, because a lack of specific mechanism of action has been confirmed. However, almost all probiotic combinations contained Streptococcus, it is therefore possible that Streptococcus cooperated with other species of probiotics are synergistic in promoting a therapeutic effect in IBS. In this study, PI-IBS mouse after gavaged with mixture of three species, ameliorated visceral sense, intestinal permeability and cytokine profiles. Compared with single species, the Mixture has, to some extent, evident advantages. According to the expression of occludin, the Mixture group was higher than Lactobacillus. In addition, the Mixture group showed decreased expression of IL-17 compared to Bifidobacterium. Based on these results, we could conclude that mixture of three stains was superior to single species. VSL#3, probiotic ‘cocktail’, was reported to be a novel probiotic for the treatment of IBS. In IBS patients with predominant bloating, VSL#3 significantly reduced flatulence scores and retarded colonic transit in contrast to placebo. The comparison between single probiotic and combination probiotic was not reported before, but it turned out that combination was superior to single species in this study. Thus, we have demonstrated the superiority of mixture of three species in barrier protection as well as immunoregulation. In summary, the GDC-0449 literature confirms the benefit of Bifidobacterium and Lactobacillus alone or the combination of the three species on the gut sensation, intestinal permeability in PI-IBS mouse model; the mechanisms supporting these beneficial effects may be upregulation of tight junction proteins and restriction inflammation. Nonertheless, Streptococcus shows either no effect or a favorable effect. Most importantly, we have demonstrated the superiority of mixture of three species over a single one. This study may aid our understanding of the mechanisms underlying probiotic treatments for PI-IBS, which might offer referrences to select appropriate probitic species for IBS patients with different symptoms. The American Stroke Association estimates that stroke accounts for 1 out of every 18 deaths and occurs every 40 seconds in the United States. In the 2012 update, the majority of strokes were ischemic ; 10% were intracerebral hemorrhage; 3% were due to subarachnoid hemorrhage. Ischemic stroke results from the occlusion of a cerebral artery, leading to a blocked cerebral blood flow to certain part of the brain. Two emergent clinical therapies for acute ischemic stroke are: reperfusion of the blood flow and neuroprotection of the injured brain cells. Early reperfusion within 3 h is beneficial to improve the outcome of acute human ischemic stroke. However, late recovery of circulation might cause reperfusion injury, resulting in blood-brain barrier breakdown, or brain edema. Although many animal stroke models have been developed, no single model can fully mimic clinical human stroke because of its heterogeneity. The transient three vessels occlusion method provides a model for the study of ischemia-reperfusion injury. This method can build a stable focal infarction in the brain.